Simplified Methods for Purification of Peanut Allergenic Proteins: Ara h 1, Ara h 2, and Ara h 3
نویسندگان
چکیده
منابع مشابه
Ara h 2 and Ara h 6 have similar allergenic activity and are substantially redundant.
BACKGROUND The moderately homologous (approx. 60%) proteins Ara h 2 and Ara h 6 are the most potent peanut allergens. This study was designed to define the relative individual contributions of Ara h 2 and Ara h 6 to the overall allergenic activity of a crude peanut extract (CPE). METHODS Ara h 2 and Ara h 6 were removed from CPE by gel filtration chromatography. Ara h 2.01, Ara h 2.02 and Ara...
متن کاملAra h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut
The similarities of two major peanut allergens, Ara h 2 and Ara h 6, in molecular size, amino acid sequence, and structure have made it difficult to obtain natural Ara h 6 free of Ara h 2. The objectives of this study were to purify natural Ara h 6 that is essentially free of Ara h 2 and to compare its IgE reactivity and potency in histamine release assays to Ara h 2. Sodium dodecyl sulfate-pol...
متن کاملIgE cross-reactivity between the major peanut allergen Ara h 2 and the non-homologous allergens Ara h 1 and Ara h 3
BACKGROUND Ara h 1, a vicilin; Ara h 2, a 2S albumin; and Ara h 3, a legumin, are major peanut allergens. Ara h 2 is an important predictor of clinical reactivity to peanut, but cosensitization to all 3 allergens is correlated with the severity of patients' symptoms. OBJECTIVE We investigated whether cosensitization to these 3 allergens is caused by IgE cross-reactivity, despite the fact that...
متن کاملImpedance biosensor for peanut protein Ara h 1.
A reagentless electrochemical impedance biosensor for detection of peanut protein Ara h 1, one of the allergenic proteins found in peanuts, has been demonstrated using an Au substrate onto which an antibody film has been immobilized. Following initial stabilization of the self-assembled monolayer (SAM) through which the antibody is immobilized, the biosensor substrate exhibits stable impedance ...
متن کاملPeanut allergen (Ara h 1) detection in foods containing chocolate.
Inadvertent exposure to peanut in foods poses health risks for peanut-allergic individuals that can be reduced by improving detection systems for allergen contaminants in food products and manufacturing processes. Detection of peanut in chocolate has been especially difficult. We report the optimization of conditions for measuring a major peanut allergen, Ara h 1, in chocolate with the use of a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Food Science and Technology Research
سال: 2014
ISSN: 1344-6606,1881-3984
DOI: 10.3136/fstr.20.875